Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/126700
Title: Prostate cancer cells modulate osteoblast mineralisation and osteoclast differentiation through Id-1
Authors: Wong, YC;Chan, KW;Yuen, HF;Chiu, YT;Chan, KK;Chan, YP;Chua, CW;McCrudden, CM;Tang, KH;ElTanani, M;Wang, X
subject: Bone metastasis;Id-1;Osteoblast;Osteoclast;Prostate Cancer
Year: 2010
Publisher: Nature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
United Kingdom
Description: Background: Id-1 is overexpressed in and correlated with metastatic potential of prostate cancer. The role of Id-1 in this metastatic process was further analysed. Methods: Conditioned media from prostate cancer cells, expressing various levels of Id-1, were used to stimulate pre-osteoclast differentiation and osteoblast mineralisation. Downstream effectors of Id-1 were identified. Expressions of Id-1 and its downstream effectors in prostate cancers were studied using immunohistochemistry in a prostate cancer patient cohort (N110). Results: We found that conditioned media from LNCaP prostate cancer cells overexpressing Id-1 had a higher ability to drive osteoclast differentiation and a lower ability to stimulate osteoblast mineralisation than control, whereas conditioned media from PC3 prostate cancer cells with Id-1 knockdown were less able to stimulate osteoclast differentiation. Id-1 was found to negatively regulate TNF-Β and this correlation was confirmed in human prostate cancer specimens (P0.03). Furthermore, addition of recombinant TNF-Β to LNCaP Id-1 cell-derived media blocked the effect of Id-1 overexpression on osteoblast mineralisation. Conclusion: In prostate cancer cells, the ability of Id-1 to modulate bone cell differentiation favouring metastatic bone disease is partially mediated by TNF-Β, and Id-1 could be a potential therapeutic target for prostate cancer to bone metastasis. © 2010 Cancer Research UK. All rights reserved.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-75549090864&selection=ref&src=s&origin=recordpage
http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-0920&volume=102&spage=332&epage=341&date=2010&atitle=Prostate+Cancer+Cells+Modulate+Osteoblast+Mineralisation+And+Osteoclast+Differentiation+Through+Id-1
http://hub.hku.hk/handle/10722/126700
Standard no: British Journal Of Cancer, 2010, v. 102 n. 2, p. 332-341
6431612
10.1038/sj.bjc.6605480
341
178833
218578
WOS:000273728500013
0007-0920
2
20010941
eid_2-s2.0-75549090864
332
102
Appears in Collections:Department of Anatomy

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