Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/144508
Title: Mequindox induced cellular DNA damage via generation of reactive oxygen species
Authors: Liu, J;Ouyang, M;Jiang, J;Mu, P;Wu, J;Yang, Q;Zhang, C;Xu, W;Wang, L;Huen, MSY;Deng, Y
Year: 2012
Publisher: Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gentox
Netherlands
Description: Mequindox, a quinoxaline-N-dioxide derivative that possesses antibacterial properties, has been widely used as a feed additive in the stockbreeding industry in China. While recent pharmacological studies have uncovered potential hazardous effects of mequindox, exactly how mequindox induces pathological changes and the cellular responses associated with its consumption remain largely unexplored. In this study, we investigated the cellular responses associated with mequindox treatment. We report here that mequindox inhibits cell proliferation by arresting cells at the G2/M phase of the cell cycle. Interestingly, this mequindox-associated deleterious effect on cell proliferation was observed in human, pig as well as chicken cells, suggesting that mequindox acts on evolutionarily conserved target(s). To further understand the mequindox-host interaction and the mechanism underlying mequindox-induced cell cycle arrest, we measured the cellular content of DNA damage, which is known to perturb cell proliferation and compromise cell survival. Accordingly, using γ-H2AX as a surrogate marker for DNA damage, we found that mequindox treatment induced cellular DNA damage, which paralleled the chemical-induced elevation of reactive oxygen species (ROS) levels. Importantly, expression of the antioxidant enzyme catalase partially alleviated these mequindox-associated effects. Taken together, our results suggest that mequindox cytotoxicity is attributable, in part, to its role as a potent inducer of DNA damage via ROS. © 2011 Elsevier B.V.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-83955161129&selection=ref&src=s&origin=recordpage
http://hub.hku.hk/handle/10722/144508
Standard no: Mutation Research - Genetic Toxicology And Environmental Mutagenesis, 2012, v. 741 n. 1-2, p. 70-75
10014603
10.1016/j.mrgentox.2011.10.012
75
198264
WOS:000300746800008
1383-5718
1-2
22094289
eid_2-s2.0-83955161129
70
741
Appears in Collections:Department of Anatomy

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