Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/148384
Title: Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome: A differential expression study
Authors: Ngan, HYS;Chan, KYK;Khoo, US;Tsao, SW;Cheung, ANY;Fong, PY;Xue, WC;Chiu, PM;Man, LS
subject: c-DNA array;Gestational trophoblastic disease;Hydatidiform mole;Mcl-1
Year: 2005
Publisher: John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
United States
Description: BACKGROUND. Hydatidiform moles (HMs) are abnormal pregnancies with a propensity for developing persistent disease in the form of gestational trophoblastic neoplasia (GTN), which requires chemotherapy. In previous studies, the authors demonstrated that low apoptotic activity was correlated with the progression of HM to GTN, and they hypothesized that some apoptosis-related genes may determine this progression. METHODS. The differential expression of apoptotic genes in HMs that subsequently developed into GTN was compared with the same expression in HMs that spontaneously regressed using a human apoptosis array; then, the expression was evaluated with real-time quantitative polymerase chain reaction analysis and immunohistochemistry using 54 clinical samples from patients with HMs who had follow-up data available. RESULTS. Using an apoptosis array, greater expression of Mcl-1, which is an antiapoptotic gene, was detected in HMs that subsequently developed into GTN. It was confirmed that the levels of Mcl-1 RNA expression (P = 0.017) and Mcl-1 protein expression (P < 0.001) in HMs that developed into persistent disease and required chemotherapy were significantly greater compared with the levels in HMs that regressed. Moreover, Mcl-1 immunoreactivity, which was detected predominantly in cytotrophoblasts, was correlated with the apoptotic index, as assessed with M30 cytoDeath immunohistochemistry, which is a good indicator of apoptotic events in the early-stage disease. CONCLUSIONS. The current results demonstrated that Mcl-1, as identified by a cyclic DNA array, may play a role in the pathogenesis of HMs and may have potential as a useful marker for predicting the clinical behavior of HMs. © 2004 American Cancer Society.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-12344276322&selection=ref&src=s&origin=recordpage
http://hub.hku.hk/handle/10722/148384
Standard no: Cancer, 2005, v. 103 n. 2, p. 268-276
10.1002/cncr.20767
276
109806
WOS:000226379000008
0008-543X
2
15578716
eid_2-s2.0-12344276322
268
103
Appears in Collections:Department of Anatomy

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