Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/149617
Title: Alterations of biologic properties and gene expression in nasopharyngeal epithelial cells by the Epstein-Barr virus-encoded latent membrane protein 1
Authors: Lo, AKF;Liu, Y;Wang, XH;Huang, DP;Yuen, PW;Wong, YC;Tsao, GSW
Year: 2003
Publisher: Nature Publishing Group. The Journal's web site is located at http://www.nature.com/labinvest/
United Kingdom
Description: Undifferentiated nasopharyngeal carcinoma (NPC) is closely associated with EBV infection, and the EBV-encoded latent membrane protein 1 (LMP1) is frequently detected in NPC. However, little is known about the pathologic roles of LMP1 in this disease. Recently, we reported the morphologic transformation and increased expression of the LAMC2 and ITGα6 genes in LMP1-expressing NPC cell lines. In this study, we further examine the effects of LMP1 in an immortalized nasopharyngeal epithelial cell line called NP69. This cell line was established from primary nonmalignant nasopharyngeal epithelial cells and may represent a model of premalignant nasopharyngeal epithelial cells. LMP1 induced many phenotypic changes in NP69 cells. These include morphologic transformation, increased cell proliferation, anchorage-independent growth, resistance to serum free-induced cell death, and enhanced cell migration and invasion. In addition, expression array analysis identified 28 genes that demonstrated a more than 2-fold difference in expression of NP69 cells expressing LMP1 when compared with a vector control. Two of the up-regulated genes (VEGF and vimentin) identified have been previously reported as LMP1 targets. The majority of the identified genes are associated with cell growth, differentiation, cell shape, and invasion. The present findings support the proposed roles of LMP1 in promoting cell transformation, migration, and invasion in premalignant nasopharyngeal epithelial cells. The present study also indicates the activation of the Ras/MAPK pathway in LMP1-expressing cells, which may be involved in mediating some of the transforming effects of LMP1 observed in nasopharyngeal epithelial cells.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-0038814052&selection=ref&src=s&origin=recordpage
http://hub.hku.hk/handle/10722/149617
Standard no: Laboratory Investigation, 2003, v. 83 n. 5, p. 697-709
709
76480
WOS:000183114700010
0023-6837
5
12746479
eid_2-s2.0-0038814052
697
83
Appears in Collections:Department of Anatomy

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