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Title: A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
Authors: Koo, CK;Wong, KL;Man, CWY;Lam, YW;So, LKY;Tam, HL;Tsao, SW;Cheah, KW;Lau, KC;Yang, YY;Chen, JC;Lam, MHW
Year: 2009
Publisher: American Chemical Society. The Journal's web site is located at
United States
Description: The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.
Standard no: Inorganic Chemistry, 2009, v. 48 n. 3, p. 872-878
Appears in Collections:Department of Anatomy

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