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|Title:||Highly restricted expression of Cre recombinase in cerebellar Purkinje cells|
|Authors:||Zhang, XM;Ng, AHL;Tanner, JA;Wu, WT;Copeland, NG;Jenkins, NA;Huang, JD|
|subject:||BAC;Cerebellum;Cre recombinase;L7;Pcp2;Purkinje cells;Transgenic mouse|
|Publisher:||John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/68503812|
|Description:||The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. © 2004 Wiley-Liss, Inc.|
|Standard no:||Genesis, 2004, v. 40 n. 1, p. 45-51|
|Appears in Collections:||Department of Anatomy|
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