Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/67488
Title: Differential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray
Authors: Tsao, SW;Ngan, HYS;Chan, KW;Cheung, ANY;Feng, HC;Kwan, HS;Shih, SM;Xue, WC;Chiu, PM
subject: cDNA microarray;Differential gene expression;Hydatidiform mole;Suppression subtractive hybridization
Year: 2006
Publisher: WB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/placenta
United Kingdom
Description: Complete hydatidiform mole (CHM) is a type of gestational trophoblastic disease with pure paternal chromosome contribution and unpredictable malignant potential. As an attempt to assess the molecular pathogenesis of CHM, suppression subtractive hybridization (SSH) combined with cDNA microarray was used to compare the gene expression pattern of CHM compared with normal first-trimester placenta of similar gestational ages. cDNA microarray analysis using tissue-specific chips constructed with subtracted cDNA libraries identified 13 differentially expressed gene transcripts. Quantitative real-time polymerase chain reaction (PCR) confirmed up-regulation of human chorionic gonadotropin β subunit (CGB) (P = 0.0008) and KIAA1200 (P = 0.0005), a G-protein regulator, as well as down-regulation of osteopontin (SPP1) (P < 0.0001) in 14 genotyped CHM when compared with 15 normal placentas. These candidate genes may contribute toward understanding the mechanism involved with the development and progression of CHM. © 2005 Elsevier Ltd. All rights reserved.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-33645080437&selection=ref&src=s&origin=recordpage
http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0143-4004&volume=27&spage=521&epage=526&date=2006&atitle=Differential+gene+expression+identified+in+complete+hydatidiform+mole+by+combining+suppression+subtractive+hybridization+and+cDNA+microarray
http://hub.hku.hk/handle/10722/67488
Standard no: Placenta, 2006, v. 27 n. 4-5, p. 521-526
10.1016/j.placenta.2005.05.005
526
109888
WOS:000237329100023
0143-4004
4-5
16026829
eid_2-s2.0-33645080437
521
27
Appears in Collections:Department of Anatomy

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