Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/67519
Title: A New Method for Improving Metaphase Chromosome Spreading
Authors: Deng, W;Tsao, SW;Cheung, ALM;Lucas, JN;Leung, CS
subject: Chromosome spreading;Dynamics;Mechanism;New method
Year: 2003
Publisher: John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0196-4763/
United States
Description: Background: The success of complex molecular cytogenetic studies depends on having properly spread chromosomes. However, inconsistency of optimum chromosome spreading remains a major problem in cytogenetic studies. Methods: The metaphase spreading process was carefully timed to identify the most critical phase of chromosome spreading. The effects of dropping height of cell suspension, slide condition, drying time, fixative ratio, and relative humidity on the quality of metaphase spreads were studied by quantitative examination of metaphase chromosome spreads. Normal and immortalized human epithelial ovarian cells, neuroblastoma cells, and normal lymphocytes were tested. Results: Humidity over the slide was the most important variable affecting the quality of chromosome spreads. Consistent improvement in chromosome spreading (larger metaphase area, less chromosome overlaps, or lower frequencies of broken metaphases) was obtained for all cell types if dynamic cell rehydration, occurring as fixative absorbs moisture from air, was made to coincide with the prompt fixation of spread chromosomes to the slide. This was achieved by dropping cells on dry glass slides placed in a shallow metal tray and then quickly lowering the tray into a covered 50°C water bath for slide drying. Conclusions: A new and simple method for improving metaphase chromosome spreading was developed based on our study on the characteristics of chromosome spreading. © 2002 Wiley-Liss, Inc.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-0345168793&selection=ref&src=s&origin=recordpage
http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0196-4763&volume=51A&spage=46&epage=51&date=2003&atitle=A+New+Method+for+Improving+Metaphase+Chromosome+Spreading
http://hub.hku.hk/handle/10722/67519
Standard no: Cytometry Part A, 2003, v. 51 n. 1, p. 46-51
51
75683
WOS:000182425600006
1552-4922
1
12500304
eid_2-s2.0-0345168793
46
51
Appears in Collections:Department of Anatomy

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