Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/67627
Title: Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons
Authors: Tanner, JA;Tay, D;So, KF;Huang, JD;Zhang, XM;Chen, BY;Ng, AHL;Rachel, RA;Copeland, NG;Jenkins, NA
Year: 2005
Publisher: Association for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
United States
Description: PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation. Copyright © Association for Research in Vision and Ophthalmology.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-32944457757&selection=ref&src=s&origin=recordpage
http://hub.hku.hk/handle/10722/67627
Standard no: Investigative Ophthalmology And Visual Science, 2005, v. 46 n. 10, p. 3515-3520
10.1167/iovs.04-1201
3520
107078
WOS:000232112900009
0146-0404
10
16186328
eid_2-s2.0-32944457757
3515
46
Appears in Collections:Department of Anatomy

Files in This Item:
Click on the URI links for accessing contents.


Items in HannanDL are protected by copyright, with all rights reserved, unless otherwise indicated.