Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/90837
Title: 2-Ammonio-6-(3-oxidopyridinium-1-yl)hexanoate (OP-lysine) Is a Newly Identified Advanced Glycation End Product in Cataractous and Aged Human Lenses
Authors: Argirov, OK;Lin, B;Ortwerth, BJ
subject: Species Index: Animalia
Year: 2004
Publisher: American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Description: Post-translational modifications of proteins take place during the aging of human lens. The present study describes a newly isolated glycation product of lysine, which was found in the human lens. Cataractous and aged human lenses were hydrolyzed and fractionated using reverse-phase and ion-exchange high performance liquid chromatography (HPLC). One of the non-proteinogenic amino acid components of the hydrolysates was identified as a 3-hydroxypyridinium derivative of lysine, 2-ammonio-6-(3-oxidopyridinium-1-yl)hexanoate (OP-lysine). The compound was synthesized independently from 3-hydroxypyridine and methyl 2-[(tert-butoxycarbonyl)amino]-6-iodohexanoate. The spectral and chromatographic properties of the synthetic OP-lysine and the substance isolated from hydrolyzed lenses were identical. HPLC analysis showed that the amounts of OP-lysine were higher in water-insoluble compared with water-soluble proteins and was higher in a pool of cataractous lenses compared with normal aged lenses, reaching 500 pmol/mg protein. The model incubations showed that an anaerobic reaction mixture of Nα-tert-butoxycarbonyllysine, glycolaldehyde, and glyceraldehyde could produce the Nα -t-butoxycarbonyl derivative of OP-lysine. The irradiation of OP-lysine with UVA under anaerobic conditions in the presence of ascorbate led to a photochemical bleaching of this compound. Our results argue that OP-lysine is a newly identified glycation product of lysine in the lens. It is a marker of aging and pathology of the lens, and its formation could be considered as a potential cataract risk-factor based on its concentration and its photochemical properties.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-1342325434&selection=ref&src=s&origin=recordpage
http://hub.hku.hk/handle/10722/90837
Standard no: Journal of Biological Chemistry, 2004, v. 279 n. 8, p. 6487-6495
10.1074/jbc.M309090200
6495
WOS:000188969200034
0021-9258
8
14634019
eid_2-s2.0-1342325434
6487
279
Appears in Collections:Department of Anatomy

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