Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/90918
Title: Relationships between Microbial Community Structure and Hydrochemistry in a Landfill Leachate-Polluted Aquifer
Authors: Röling, WFM;Van Breukelen, BM;Braster, M;Lin, B;Van Verseveld, HW
subject: Chemicals And Cas Registry Numbers
Year: 2001
Publisher: American Society for Microbiology
Description: Knowledge about the relationship between microbial community structure and hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill leachate-polluted aquifers is required to develop tools for predicting and monitoring natural attenuation. In this study analyses of pollutant and redox chemistry were conducted in parallel with culture-independent profiling of microbial communities present in a well-defined aquifer (Banisveld, The Netherlands). Degradation of organic contaminants occurred under iron-reducing conditions in the plume of pollution, while upstream of the landfill and above the plume denitrification was the dominant redox process. Beneath the plume iron reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea in 29 groundwater samples revealed a clear difference between the microbial community structures inside and outside the contaminant plume. A similar relationship was not evident in sediment samples. DGGE data were supported by sequencing cloned 16S rDNA. Upstream of the landfill members of the β subclass of the class Proteobacteria (β-proteobacteria) dominated. This group was not encountered beneath the landfill, where gram-positive bacteria dominated. Further downstream the contribution of gram-positive bacteria to the clone library decreased, while the contribution of δ-proteobacteria strongly increased and β-proteobacteria reappeared. The β-proteobacteria (Acidovorax, Rhodoferax) differed considerably from those found upstream (Gallionella, Azoarcus). Direct comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data from each analysis were comparable. A relationship was observed between the dominant redox processes and the bacteria identified. In the iron-reducing plume members of the family Geobacteraceae made a strong contribution to the microbial communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated aquifers deserves more detailed consideration.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-0035491310&selection=ref&src=s&origin=recordpage
http://hub.hku.hk/handle/10722/90918
Standard no: Applied and Environmental Microbiology, 2001, v. 67 n. 10, p. 4619-4629
10.1128/AEM.67.10.4619-4629.2001
4629
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WOS:000171237700031
0099-2240
10
PMC93212
11571165
eid_2-s2.0-0035491310
4619
67
Appears in Collections:Department of Anatomy

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