Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/10722/90940
Title: Restoration of visual function in retinal degeneration mice by ectopic expression of melanopsin
Authors: Lin, B;Koizumi, A;Tanaka, N;Panda, S;Masland, RH
subject: Species Index: Bacteria (Microorganisms);Mammalia;Mus
Year: 2008
Publisher: National Academy of Sciences. The Journal's web site is located at http://www.pnas.org
United States
Description: The rod and cone cells of the mammalian retina are the principal photoreceptors for image-forming vision. They transmit information by means of a chain of intermediate cells to the retinal ganglion cells, which in turn send signals from the retina to the brain. Loss of photoreceptor cells, as happens in a number of human diseases, leads to irreversible blindness. In a mouse model (rd/rd) of photoreceptor degeneration, we used a viral vector to express in a large number of retinal ganglion cells the light sensitive protein melanopsin, normally present in only a specialized subset of the cells. Whole-cell patch-clamp recording showed photoresponses in these cells even after degeneration of the photoreceptors and additional pharmacological or Cd 2+ block of synaptic function. Interestingly, similar responses were observed across a wide variety of diverse types of ganglion cell of the retina. The newly melanopsin-expressing ganglion cells provided an enhancement of visual function in rd/rd mice: the pupillary light reflex (PLR) returned almost to normal; the mice showed behavioral avoidance of light in an open-field test, and they could discriminate a light stimulus from a dark one in a two-choice visual discrimination alley. Recovery of the PLR was stable for at least 11 months. It has recently been shown that ectopic retinal expression of a light sensitive bacterial protein, channelrhodopsin-2, can restore neuronal responsiveness and simple visual abilities in rd/rd mice. For therapy in human photodegenerations, channelrhodopsin-2 and melanopsin have different advantages and disadvantages; both proteins (or modifications of them) should be candidates. © 2008 by The National Academy of Sciences of the USA.
URI: http://www.scopus.com/mlt/select.url?eid=2-s2.0-57349113014&selection=ref&src=s&origin=recordpage
http://hub.hku.hk/handle/10722/90940
Standard no: Proceedings Of The National Academy Of Sciences Of The United States Of America, 2008, v. 105 n. 41, p. 16009-16014
10.1073/pnas.0806114105
1091-6490
16014
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
1123390
228172
WOS:000260240900066
0027-8424
41
PMC2572922
18836071
eid_2-s2.0-57349113014
16009
105
Appears in Collections:Department of Anatomy

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