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|Title:||Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts|
|Authors:||Mehta, IS;Amira, M;Harvey, AJ;Bridger, JM|
|Description:||Background: Radial chromosome positioning in interphase nuclei is nonrandom and
can alter according to developmental, differentiation, proliferation, or disease status.
However, it is not yet clear when and how chromosome repositioning is elicited.
Results: By investigating the positioning of all human chromosomes in primary
fibroblasts that have left the proliferative cell cycle, we have demonstrated that in
cells made quiescent by reversible growth arrest, chromosome positioning is altered
considerably. We found that with the removal of serum from the culture medium,
chromosome repositioning took less than 15 minutes, required energy and was
inhibited by drugs affecting the polymerization of myosin and actin. We also
observed that when cells became quiescent, the nuclear distribution of nuclear myosin
1ß was dramatically different from that in proliferating cells. If we suppressed the
expression of nuclear myosin 1ß by using RNA-interference procedures, the
movement of chromosomes after 15 minutes in low serum was inhibited. When high
serum was restored to the serum-starved cultures, chromosome repositioning was
evident only after 24 to 36 hours, and this coincided with a return to a proliferating
distribution of nuclear myosin 1ß.
Conclusions: These findings demonstrate that genome organization in interphase
nuclei is altered considerably when cells leave the proliferative cell cycle and that
repositioning of chromosomes relies on efficient functioning of an active nuclear
motor complex that contains nuclear myosin 1ß.|
Brunel Open Access Publishing Fund
|Standard no:||Genome Biology . 11:R5|
|Appears in Collections:||Dept of Life Sciences Research Papers|
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