Please use this identifier to cite or link to this item: http://dl.umsu.ac.ir/handle/Hannan/22819
Title: Studies In Enzyme Make-up of Vibrio cholerae: Part XII-Ribonuclease Activity
Authors: Sagar, P;Krishna Murti, C R;Shrivastava, D L
subject: Vibrio cholerae;Ribonuclease;Potassturn chloride
Year: 1958
Description: Ribonucleic acid depolynlerizing activity in certain cell-free preparations and resting cell suspensions of Vibrio cholerae has been Investigated.Though the intact cells are entirely inactive, crude extracts obtained by grinding the cells with glass powder and extracting with 0·9 per cent potassium chloride solution are highly active. More than 68 per cent of the activity has been found associated with supernatants obtained by centrifugation at 73,400 g. The sedimented particulate rnatertal also possesses appreciable activity. Depolymerization of RNA by cell-free extracts is optimum at pH 8·0. The reaction is very strongly inhibited by copper ions and to a lesser extent by EDTA and 8-hydroxyquinoline. The enzyrne in cell-free extracts is inactivated by heating. The activity does not vary appreciably in the crude cell extracts of different antigenic strains of V. cholerae.
URI: http://hdl.handle.net/123456789/927
Standard no: Journal of Scientific and Industrial Research 1958,17C,196-200
Appears in Collections:Biochemistry

Files in This Item:
Click on the URI links for accessing contents.


Items in HannanDL are protected by copyright, with all rights reserved, unless otherwise indicated.