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Title: High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons
Authors: Nieland, Thomas J. F.;Logan, David J.;Saulnier, Jessica;Lam, Daniel;Johnson, Caroline;Root, David E.;Carpenter, Anne E.;Sabatini, Bernardo L.
subject: Biology and Life Sciences;Anatomy;Nervous System;Neuroanatomy;Connectomics;Genetics;Genomics;Functional Genomics;Neuroscience;Cellular Neuroscience;Neuronal Morphology;Developmental Neuroscience;Computer and Information Sciences;Software Engineering;Software Tools;Engineering and Technology;Specimen Preparation and Treatment;Mechanical Treatment of Specimens;Specimen Disruption;Electroporation
Year: 2014
Publisher: Public Library of Science
Description: The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders.
Standard no: Nieland, Thomas J. F., David J. Logan, Jessica Saulnier, Daniel Lam, Caroline Johnson, David E. Root, Anne E. Carpenter, and Bernardo L. Sabatini. 2014. “High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons.” PLoS ONE 9 (3): e91744. doi:10.1371/journal.pone.0091744.
Appears in Collections:HMS Scholarly Articles

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